FLOWJO DEPARTMENTAL LICENSE SOFTWARE
FLOWJO DEPARTMENTAL LICENSE LICENSE

Using the high-resolution camera and analytical software, which can be applied for studying the environmental toxicity on microbiota. (iv) The growth of microbes from various pathogenic sources or from complex microbiota pools can be recorded, quantified, and differentiated (iii) Many reagents kits have been commercially developedįor testing cytokine production that can be conveniently applied in toxicology studies. regulatory T cells, anti-inflammatory immune cells, etc, from human samples in response to toxicant-treatment or environmental exposure.

(ii) Functional measurement of the cytokine production from rare (i) Characterization of cytokine, surface markers, or soluble molecules from rare cancer stem cells with high sensitivity and accuracy to capture individual cells. This ImmunoSpot ® S6 Micro Analyzer can be applied to the following CEG-related or supported studies involving in environmentally induced diseases and conditions. It is a low maintenance and high performance instrument for measuring functional responses and clustering growth derivedįrom single cells at a high-throughput plate-based setting. S6 Micro provides the ultimate in image quality, especially for double-color assays (no fluorescence signal detected by this model). With its maintenance-free 150-diode overhead lighting system and proprietary planar backlight system, the ImmunoSpot®

Seamless switching from 6- to 384-well plates and counting objects <10 µm in size – including individual cells. Technically, the ImmunoSpot ® S6 Micro’s zoom lens permits ImmunoSpot ® Analyzers are designed for scanning and evaluating single cell-based spots, which reflect the protein expression, function, and growth of cells. Research supported by the Center for Environmental Genetics should acknowledge NIEHS Award P30 ES006096. Huang to discuss experimental design, pitfalls, and data analyses.analyses. Currently, the service is open every week for two-three hours slots on Monday and Wednesday afternoons.Ĭonsultation Users are encouraged to make appointments with Dr. This service is currently offered at restricted time. Staff-operated assays A staff is available to run pre-prepared samples. Users are responsible for the correct manipulation of the instrument to achieve results and avoid damages. Usage may be available after working hours at a case-by-case scheduling base. Trained users can reserve and access the instrument from 8:00 am to 5:00 pm, Monday through Friday. Users can request more training hours if they are unable to fully perform the assays independently. At least one-hour instrument demonstration and one-hour guided hands-on sample running are required prior to independent usage.

FLOWJO DEPARTMENTAL LICENSE SOFTWARE

Training mainly involves basic instrument and software manipulation to allow new users to start sample measurement independently. These resources include the instrument website of Guava eas圜yte 12-channel high throughput (12HT) flow cytometer, flow cytometry resources, on campus lectures provided by Ohio River Valley Cytometry Association (ORVCA), and related seminars from CEG.Ĭontact: Shouxiong Huang (Kettering Rm 248, Rm 251), Manju Sharma (Kettering Rm 248, Rm 276) The instrument is located in Rm 253 Kettering Users are encouraged to understand the basic knowledge of flow cytometry in experimental design, antibody staining, compensation, and data analyses together with unique setting for this eas圜yte unit to ensure success.

FLOWJO DEPARTMENTAL LICENSE LICENSE

Data can also be analyzed with FlowJo (group license is available from CCHMC flow cytometry core), FCS Express, and ModFit LT, which are also available for data analysis free of charge to CCHMC flow core users in R5503. If the computer space is limited, the stored data will be removed after 6-month from initial acquisition.ĭata Analysis Data can be analyzed with the software GuavaSoft inCyte or Guava Suite provided by the manufacturer. The cell suspension should be maintained at ~200 cell/ml and ~200 ul/well for the 96-well plate format.ĭata Storage A project drive, called “Document-Flow users”, has been set up for temporary storage. Sample format Samples should be prepared with 96-well sample plates (PDF) (U-bottom preferred) or 1.5ml sample tubes (PDF). These features provide advantages and cause errors meanwhile in sample measurement. A rotating and variable-speed mixing paddle is used between each well to help cells stay in suspension during sampling. The automated sampler for a 96-well plate and 10 sample tubes enables walkaway sample handling for higher throughput assays. This flow cytometer uses microcapillary to allow direct sampling by aspiration and eliminates the need of sheath fluid. Flow cytometer: The Luminex (previously Millipore) provides 3 lasers (violet, blue, and red) and 10 fluorescence channels for the detection of fluorescence-labeled cells and particles.

Flowjo departmental license